Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: ( a ) CFU counts and relative proportion (%) of E. coli and S. oneidensis strains in anode media. ( b ) CFU counts and relative proportion (%) of E. coli and S. oneidensis strains on the anode electrode.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques:

Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: Selected gene expression changes in E. coli induced by S. oneidensis for energy metabolism.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques: Gene Expression

Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: Glucose is fermented by E. coli to yield metabolites such as formate, which is taken up by S. oneidensis as electron donor. Flavin mediator molecules are secreted to facilitate electron movement to the external charge collecting electrode to derive energy in MFCs. Flavins can also be taken up by E. coli to facilitate its respiration on electrodes as E. coli does not secrete flavins.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques:

Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: Bacterial strains used in this study.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques:

Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: ( a ) Photograph of MFC setup. ( b ) Graphic of mutualistic interactions between E. coli and S. oneidensis in MFC setup.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques:

Journal: Bioorganic chemistry

Article Title: Dimeric peptoids as antibacterial agents.

doi: 10.1016/j.bioorg.2024.107334

Figure Lengend Snippet: Fig. 11. S. aureus 38 (A, B and C) and E. coli k12 (D, E, F) membrane permeabilization by peptoid 10 k at their 2XMIC determined by flow cytometry using SYTO9 (membrane permeable) and Propidium iodide (membrane impermeable) stains.

Article Snippet: The efficacy of dimer peptoids was examined against Staphylococcus aureus 38 (originally isolated from an ocular infection) and the Gramnegative bacteria Escherichia coli K12 (ATCC 10798, originally isolated from the faeces of a diphtheria convalescent patient) and Pseudomonas aeruginosa PA01 (originally isolated from a wound), by determining their minimum inhibitory concentrations (Table 1).

Techniques: Membrane, Flow Cytometry

Journal:

Article Title: Detection and Quantification of Methyl tert -Butyl Ether-Degrading Strain PM1 by Real-Time TaqMan PCR

doi: 10.1128/AEM.67.11.5154-5160.2001

Figure Lengend Snippet: Bacterial strains used to test the specificity of the PM1 TaqMan assay

Article Snippet: Genomic DNA was extracted with standard methods ( 2 ) and quantified using a Lambda 10 UV/Vis spectrometer (PE Applied Biosystems). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Medium C T value b PM1 MSM 23.2 E. coli K-12 ATCC 10798 ATCC medium 3 40 Bacillus subtilis ATCC 6051 ATCC medium 3 40 P. putida ATCC 12633 ATCC medium 3 38.1 S. lactis ATCC 19435 ATCC medium 17 38.68 Staphylococcus aureus ATCC ATCC medium 3 40 A. facilis ATCC 11228 ATCC medium 81 37.03 H. flava ATCC 33667 ATCC medium 3 40 Micrococcus luteus ATCC 4698 ATCC medium 18 40 Acinetobacter calcoaceticus ATCC medium 3 40 Azomonas agilis ATCC 7494 ATCC medium 11 40 A. commune DSMZ 11901 DSMZ medium 464 39.55 Mycobacterium sp. ATCC medium 90 40 Gr(+) bacterial isolate 0.1 × TS 40 Gr(−) bacterial isolate 0.1 × TS 40 Nitrosomonas europaea ATCC 19718 ATCC medium 221 40 Open in a separate window a Strains were obtained from American Type Culture Collection (ATCC; Manassas, Va.) or Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ; Braunschweig, Germany). b C T values obtained when 1,000 pg of DNA was used as a DNA template in the TaqMan PCR.

Techniques:

Journal:

Article Title: Detection and Quantification of Methyl tert -Butyl Ether-Degrading Strain PM1 by Real-Time TaqMan PCR

doi: 10.1128/AEM.67.11.5154-5160.2001

Figure Lengend Snippet: TaqMan PM1 detection in spiked sediment or groundwater microcosms

Article Snippet: Genomic DNA was extracted with standard methods ( 2 ) and quantified using a Lambda 10 UV/Vis spectrometer (PE Applied Biosystems). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Medium C T value b PM1 MSM 23.2 E. coli K-12 ATCC 10798 ATCC medium 3 40 Bacillus subtilis ATCC 6051 ATCC medium 3 40 P. putida ATCC 12633 ATCC medium 3 38.1 S. lactis ATCC 19435 ATCC medium 17 38.68 Staphylococcus aureus ATCC ATCC medium 3 40 A. facilis ATCC 11228 ATCC medium 81 37.03 H. flava ATCC 33667 ATCC medium 3 40 Micrococcus luteus ATCC 4698 ATCC medium 18 40 Acinetobacter calcoaceticus ATCC medium 3 40 Azomonas agilis ATCC 7494 ATCC medium 11 40 A. commune DSMZ 11901 DSMZ medium 464 39.55 Mycobacterium sp. ATCC medium 90 40 Gr(+) bacterial isolate 0.1 × TS 40 Gr(−) bacterial isolate 0.1 × TS 40 Nitrosomonas europaea ATCC 19718 ATCC medium 221 40 Open in a separate window a Strains were obtained from American Type Culture Collection (ATCC; Manassas, Va.) or Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ; Braunschweig, Germany). b C T values obtained when 1,000 pg of DNA was used as a DNA template in the TaqMan PCR.

Techniques: Standard Deviation, Control

Journal:

Article Title: Detection and Quantification of Methyl tert -Butyl Ether-Degrading Strain PM1 by Real-Time TaqMan PCR

doi: 10.1128/AEM.67.11.5154-5160.2001

Figure Lengend Snippet: PM1 detection in groundwater DNA samples collected from an MTBE-contaminated plume at Port Hueneme, California

Article Snippet: Genomic DNA was extracted with standard methods ( 2 ) and quantified using a Lambda 10 UV/Vis spectrometer (PE Applied Biosystems). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Medium C T value b PM1 MSM 23.2 E. coli K-12 ATCC 10798 ATCC medium 3 40 Bacillus subtilis ATCC 6051 ATCC medium 3 40 P. putida ATCC 12633 ATCC medium 3 38.1 S. lactis ATCC 19435 ATCC medium 17 38.68 Staphylococcus aureus ATCC ATCC medium 3 40 A. facilis ATCC 11228 ATCC medium 81 37.03 H. flava ATCC 33667 ATCC medium 3 40 Micrococcus luteus ATCC 4698 ATCC medium 18 40 Acinetobacter calcoaceticus ATCC medium 3 40 Azomonas agilis ATCC 7494 ATCC medium 11 40 A. commune DSMZ 11901 DSMZ medium 464 39.55 Mycobacterium sp. ATCC medium 90 40 Gr(+) bacterial isolate 0.1 × TS 40 Gr(−) bacterial isolate 0.1 × TS 40 Nitrosomonas europaea ATCC 19718 ATCC medium 221 40 Open in a separate window a Strains were obtained from American Type Culture Collection (ATCC; Manassas, Va.) or Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ; Braunschweig, Germany). b C T values obtained when 1,000 pg of DNA was used as a DNA template in the TaqMan PCR.

Techniques: Standard Deviation

Journal:

Article Title: Detection and Quantification of Methyl tert -Butyl Ether-Degrading Strain PM1 by Real-Time TaqMan PCR

doi: 10.1128/AEM.67.11.5154-5160.2001

Figure Lengend Snippet: PM1 standard curve. Real-time analysis of serial 10-fold dilutions of PM1 DNA (A) or PM1 cells (B). The CT values are plotted against the corresponding PM1 cell numbers in the PCR. Template DNA was extracted from 1.8 × 107 CFU of PM1 corresponding to 3 × 106 CFU/PCR and was diluted 10-fold (A). For the second curve (B) DNA was extracted from serial dilutions of PM1 cells mixed with E. coli cells until 108 total CFU/ml was reached.

Article Snippet: Genomic DNA was extracted with standard methods ( 2 ) and quantified using a Lambda 10 UV/Vis spectrometer (PE Applied Biosystems). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Medium C T value b PM1 MSM 23.2 E. coli K-12 ATCC 10798 ATCC medium 3 40 Bacillus subtilis ATCC 6051 ATCC medium 3 40 P. putida ATCC 12633 ATCC medium 3 38.1 S. lactis ATCC 19435 ATCC medium 17 38.68 Staphylococcus aureus ATCC ATCC medium 3 40 A. facilis ATCC 11228 ATCC medium 81 37.03 H. flava ATCC 33667 ATCC medium 3 40 Micrococcus luteus ATCC 4698 ATCC medium 18 40 Acinetobacter calcoaceticus ATCC medium 3 40 Azomonas agilis ATCC 7494 ATCC medium 11 40 A. commune DSMZ 11901 DSMZ medium 464 39.55 Mycobacterium sp. ATCC medium 90 40 Gr(+) bacterial isolate 0.1 × TS 40 Gr(−) bacterial isolate 0.1 × TS 40 Nitrosomonas europaea ATCC 19718 ATCC medium 221 40 Open in a separate window a Strains were obtained from American Type Culture Collection (ATCC; Manassas, Va.) or Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ; Braunschweig, Germany). b C T values obtained when 1,000 pg of DNA was used as a DNA template in the TaqMan PCR.

Techniques:

Journal:

Article Title: Detection and Quantification of Methyl tert -Butyl Ether-Degrading Strain PM1 by Real-Time TaqMan PCR

doi: 10.1128/AEM.67.11.5154-5160.2001

Figure Lengend Snippet: TaqMan-estimated PM1 cell density and aerobic MTBE biodegradation in mineral media, inoculated groundwater, and groundwater collected from the bioaugmentation study at Port Hueneme. Biodegradation of MTBE in microcosms of mineral media (A) or groundwater from Port Hueneme (well CBC61CS located upstream from the field plots [B] or well B32D from the field test plot [C]), with or without addition of 106 CFU of PM1 per ml. Ave, average.

Article Snippet: Genomic DNA was extracted with standard methods ( 2 ) and quantified using a Lambda 10 UV/Vis spectrometer (PE Applied Biosystems). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Medium C T value b PM1 MSM 23.2 E. coli K-12 ATCC 10798 ATCC medium 3 40 Bacillus subtilis ATCC 6051 ATCC medium 3 40 P. putida ATCC 12633 ATCC medium 3 38.1 S. lactis ATCC 19435 ATCC medium 17 38.68 Staphylococcus aureus ATCC ATCC medium 3 40 A. facilis ATCC 11228 ATCC medium 81 37.03 H. flava ATCC 33667 ATCC medium 3 40 Micrococcus luteus ATCC 4698 ATCC medium 18 40 Acinetobacter calcoaceticus ATCC medium 3 40 Azomonas agilis ATCC 7494 ATCC medium 11 40 A. commune DSMZ 11901 DSMZ medium 464 39.55 Mycobacterium sp. ATCC medium 90 40 Gr(+) bacterial isolate 0.1 × TS 40 Gr(−) bacterial isolate 0.1 × TS 40 Nitrosomonas europaea ATCC 19718 ATCC medium 221 40 Open in a separate window a Strains were obtained from American Type Culture Collection (ATCC; Manassas, Va.) or Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ; Braunschweig, Germany). b C T values obtained when 1,000 pg of DNA was used as a DNA template in the TaqMan PCR.

Techniques: